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Food Science
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STEPHEN J. KNABEL

Professor of Food Science

437 Food Science Building
University Park, PA 16802


Ph: (814) 863-1372
FAX: (814) 863 6132

Email:
sjk9@psu.edu

 

Education | Research Interests | Recent Publications | Selected Funding Sources | Complete 6-Page Vitae (pdf)



Education:

Iowa State University, Microbiology and Food Technology, Ph.D., 1989
Iowa State University, Food Technology, M.S., 1986
Iowa State University, Food Technology, B.S., 1973

 
Research Interests:
Detection, tracking and control of foodborne pathogens: Listeria monocytogenes, E. coli O157:H7, Salmonella and Campylobacter. Development of novel methods for the recovery of injured foodborne pathogens and detection by conventional and molecular methods. Contamination of raw animal foods by gram-negative foodborne pathogens and destruction by high pH. Understanding and enhancing the mechanisms of action of foodgrade microbial inhibitors. Metal ion interactions with spoilage and pathogenic microorganisms. Role of heat shock proteins in induced thermotolerance.
 
Recent Publications:

Knabel, S. J., H. W. Walker, P. A. Hartman, and A. F. Mendonca. 1990. Effects of growth temperature and strictly anaerobic recovery on the survival of Listeria monocytogenes during pasteurization. Appl. Environ. Microbiol. 56:370-376.

Knabel, S. J., H. W. Walker, and P. A. Hartman. 1991. Inhibition of Aspergillus flavus and selected Gram-positive bacteria by chelation of essential metal cations by polyphosphates. J. Food Protect. 54:360-365.

Kim, J. W., S. J. Knabel, and S. Doores. 1993. Penetration of Salmonella typhimurium into turkey skin. J. Food Prot. 56:292-296.

Catalano, C. R., and S. J. Knabel. 1994. Incidence of Salmonella in Pennsylvania egg processing plants and destruction by high pH. J. Food Prot. 57: 587-591.

Catalano, C. R., and S. J. Knabel. 1994. Destruction of Salmonella enteritidis by high pH and rapid chilling during simulated commercial egg processing. J. Food Prot. 57:592-595.

Karr, K. J., A. N. Maretzki, and S. J. Knabel. 1994. Meat and poultry companies assess USDA's Hazard Analysis and Critical Control Point system. Food Technol. 48:117-122.

Knabel, S. J., and S. A. Thielen. 1994. Enhanced recovery of severely heat-injured, thermotolerant Listeria monocytogenes from USDA and FDA primary enrichment media using a novel, simple, strictly anaerobic method. J. Food Prot. 58:29-34.

Mendonca, A. F., and S. J. Knabel. 1994. A novel one-step enrichment system incorporating lithium for the detection of severely heat-injured Listeria monocytogenes in milk containing background microflora. Appl. Environ. Microbiol. 60:4001-4008.

Mendonca, A. F., T. L. Amoroso, and S. J. Knabel. 1994. Destruction of gram-negative food-borne pathogens by high pH involves disruption of the cytoplasmic membrane. Appl. Environ. Microbiol. 60:4009-4014.

Knabel, S. J. 1995. Foodborne illness: role of home food handling practices (Scientific Status Summary). Food Technology 49(4):119-131.

Fajardo, T. A., R. C. Anantheswaran, V. M. Puri, and S. J. Knabel. 1995. Penetration of Salmonella enteritidis into eggs subjected to rapid cooling. J. Food Prot. 58:473-477.

Teo, Y.-L., T. J. Raynor, K. R. Ellajosyula, and S. J. Knabel. 1996. Synergistic effect of high temperature and high pH on the destruction of Salmonella enteritidis and Escherichia coli O157:H7. J. Food Prot. 59:1023-1030.

Woody, J. L., R. A. Wilson and S. J. Knabel. 1998. Screening for E. coli O157:H7 in fresh and frozen ground beef using the Difco EZ ColiTM rapid detection system. J. Food Prot. 61:110-112.

Ellajosyula, K., R. A. Wilson, S. Doores, and S. J. Knabel. 1998. Destruction of Escherichia coli O157:H7 and Salmonella typhimurium in Lebanon bologna by interaction of fermentation pH, heating temperature and time. J. Food Prot. 61:152-157.

Alavi, S. H., V. M. Puri, S. J. Knabel, R. H. Mohtar, and R. C. Whiting. 1999. Development and validation of a dynamic growth model for Listeria monocytogenes in fluid whole milk. J. Food Prot. 62:170-176.

Woody, J. L., R. A. Walsh, S. Doores, W. R. Henning, R. A. Wilson, and S. J. Knabel. 2000. Role of Bacterial Association and Penetration on Destruction of Escherichia coli O157:H7 in Beef Tissue by High pH. J. Food Prot. 63:3-11.

Teo, Y.-L. and S. J. Knabel. 2000. Development of a Simple Recovery-Enrichment System for Enhanced Detection of Heat-Injured Listeria monocytogenes in Pasteurized Milk. J. Food Prot. 63:441-444.

Suh, J.-H., and S. J. Knabel. 2000. Comparison of different reducing agents for enhanced detection of heat-injured Listeria monocytogenes. J. Food Prot. 63:1058-1063.

Suh, J.-H, and S. J. Knabel. 2001. Comparison of different enrichment broths and background flora for detection of heat-injured Listeria monocytogenes in whole milk. J. Food Prot. 64:30-36.

Chikthimmah, N., R. B. Guyer, and S. J. Knabel. 2001. Validation of a 5-Log10 reduction of Listeria monocytogenes following simulated commercial processing of Lebanon bologna in a model system. J. Food Prot. 64:873-876.

Chikthimmah, N. and S. J. Knabel. 2001. Survival of Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes in and on vacuum-packaged Lebanon bologna stored at 3.6 and 13.0°C. J. Food Prot. 64:958-963.

Teo, A. Y.-L., G. R. Ziegler, and S. J. Knabel. 2001. Optimizing detection of heat-injured Listeria monocytogenes in pasteurized milk. J. Food Prot. 64:1000-1011.

Chikthimmah, N., R. Anantheswaran, R. F. Roberts, E. W. Mills and S. J. Knabel. 2001. Influence of sodium chloride on growth of lactic acid bacteria and subsequent destruction of Escherichia coli 0157:H7 during processing of Lebanon bologna. J. Food Prot. 64:1145-1150.

Chen, H., R. C. Anantheswaran, and S. J. Knabel. 2001. Optimization of iron supplementation for enhanced detection of Salmonella enteritidis in eggs. J. Food Prot. 64:1279-1285.

Knabel, S. J. 2002. Optimized one-step, recovery-enrichment broth for enhanced detection of Listeria monocytogenes in pasteurized milk and hot dogs. J. AOAC Int. 85:501-504.

Chen, H., R. C. Anantheswaran, and S. J. Knabel. 2002. Effect of rapid cooling on the growth and penetration of Salmonella Enteritidis into egg contents. J. Food Safety 22:255-271.

Kieras, S. J., E. W. Mills, S. J. Knabel, and A. N. Maretzki. 2003. Validation of pathogen destruction during manufacture of a meat-based potato snack (Chiparoo). J. Food Processing Preservation 26:385-399.

Parameswaran, S., R. B. Guyer and S. J. Knabel. 2004. Simple oPSU Broth-Bax PCR-PicoGreen system for rapid detection of Listeria monocytogenes in pasteurized milk and hot dogs. J. Food Safety 23:233-248.

Zhang, W., B. Jayarao, and S. J. Knabel. 2004. Multi-Virulence-Locus-Sequence Typing of Listeria monocytogenes. Appl. Environ. Microbiol. 70: 913-920.

Knabel, S. J. 2004. Food biosecurity in the U.S. Microbiology Australia 25:10-16.

Zhang, W., G. Wilt, A. Hughs, and S. J. Knabel. 2004. The BAX PCR for screening Listeria monocytogenes targets a partial putative gene lmo 2234 similar to iolE. J. Food Prot. 67:1507-1511.

Bull, M. K., M. M. Hayman, C. M. Stewart, E. A. Szabo and S. J. Knabel. 2005. Effect of prior growth temperature, type of enrichment medium, and temperature and time of storage on recovery of Listeria monocytogenes following high pressure processing of milk. Int. J. Food Microbiol. 101:53-61

Zhang, W., and S. J. Knabel. 2005. Development of a multiplex PCR assay for simultaneous species- and serotype-specific detection, and DNA sequence-based subtyping of Listeria monocytogenes. J. Food Prot. 68:1907-1910.

Chen, Y., Zhang W. and S. J. Knabel. 2005. Multi-virulence-locus sequence typing clarifies the epidemiology of two recent listeriosis outbreaks. J. Clin. Microbiol. 43:5291-5294.

 
Selected Funding Sources:
 Grant on Milk Safety Program, Pennsylvania Department of Agriculture Research
Grant Program, Various Food Industry Grants

For more information contact:

Juanita Wolfe, Graduate and Undergraduate Program Contact
203 Food Science Building

University Park, PA 16802

Ph: (814) 863 8667
Email:jmw5@psu.edu

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April 14, 2008
College ofAgricultural Sciences